IRF-1 can induce global splicing changes in the interleukin family of genes. (A) Array of cytokine-network IFN-associated genes and endogenous control genes are indicated. (B) Interleukin-associated genes. Total RNAs from MDA-MB-468 cells were isolated, converted to cDNA and subjected to qRT-PCR. Data are expressed as ratio of cytokine gene expression to endogenous control gene signal intensities after culturing for 24 h. All assays are plated in triplicate. *, p < 0.05, **, p < 0.01; ***, p < 0.001 versus Ad-null control.