ENO1 controls the expression of cell cycle and EMT-associated genes in glioma via PI3K/Akt pathway. (A). Knocking down endogenous ENO1 expression reduced the expression of pRb (Ser 780), NF-κB, and oncogenic cell cycle regulators including Cyclin D1 and Cyclin E1. However, total Rb and E2F1 were not affected. (B). Suppressing ENO1 expression decreased the expression of EMT-marker genes including Snail, β-catenin, Vimentin, Slug and N-cadherin but enhanced E-cadherin expression. (C). Reduced ENO1 expression depressed the expression of phos-PI3K, and Akt, but not their total protein levels. (D). Western blot analyses of E-cadherin, Cyclin D1, p-Rb in glioma U251 and U87 cells after LY294002 treatment. Each experiment was repeated three times.