Figure 5
From: Autophagic flux determines cell death and survival in response to Apo2L/TRAIL (dulanermin)
Impaired autophagic flux causes apoptosis in PCa cells by caspase-8 activation. (A) Western blot analysis for caspase-8 activation following TR. β-actin served as loading control. (B) Clonogenic survival in PC3 cells stably expressing non-target shRNA, shATG7, or shLAMP2, after TR (40 ng/ml) ± caspase-8 inhibitor z-IETD-fmk (10 μM) for 24 h (*p < 0.001). Western blot analysis of the indicated proteins in (C) C4-2 cells following TR ± CQ, and (D) PC3 cells following TR ± 3-MA or (E) PC3 cells expressing non-target siRNA or siBECN1 following TR.