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Figure 5 | Molecular Cancer

Figure 5

From: miR-630 targets IGF1R to regulate response to HER-targeting drugs and overall cancer cell progression in HER2 over-expressing breast cancer

Figure 5

miR-630’s mechanism of action. IGF1R was predicted as a direct target of miR-630. (A) (i) Initially the expression of IGF1R, HER2 and EGFR was assessed in our acquired lapatinib-resistant cells (HCC1954-LR and SKBR3-LR) and was found to be increased compared to levels in their age-matched parent cells. (ii) Inhibition of miR-630 induced an increase in IGF1R, HER2 and EGFR expression in both HCC1954-Ag and SKBR3-Ag cells compared to levels in the negative control transfected cells. (iii) Conversely, induced expression of miR-630 in lapatinib-resistant HCC1954-LR and SKBR3-LR cells caused a decrease in IGF1R, HER2 and EGFR levels. (B) The phosphorylated forms of IGF1R, HER2 and EGFR were assessed using ELISAs, indicating that (i) inhibition of miR-630 induced an increase in pIGF1R, pHER2 and pEGFR, while (ii) over-expression of miR-630 decreased the pIGF1R, pHER2 and pEGFR levels. Representative immunoblots of n = 3 biological repeats. n = 3 ± SEM, where * p <0.05, ** p <0.01, ***p < 0.001.

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Molecular Cancer

ISSN: 1476-4598