Figure 8
Photoactivated porphyrins degrade mRNA . (A) Photocleavage of RNA fragments from KRAS mRNA in three different conformations: quaduplex utr-2; single stranded (ss-RNA) and double stranded (ds-RNA) after treatment with porphyrin P2, P4 or C14 at ratios 1:1, 1:3 and 1:6 and light (7.2 J/cm2). The histograms show the percentage of clevage. Untreated RNA (lane 1), RNA treated with porphyrin only (lane 2), RNA treated with light only (lane 14), RNA treated with both porphyrin and light (lanes 3–13); (B) Quantitative RT-PCR showing the level of KRAS mRNA in B78-H1 cells after treated with porphyrin P2, P4 (250 and 500 nM) and C14 (15 and 30 nM) and light (7.2J/cm2). RT-PCR analysis performed 1 h after irradiation. The experiment has been carried out in triplicate.