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Figure 4 | Molecular Cancer

Figure 4

From: F-box protein complex FBXL19 regulates TGFβ1-induced E-cadherin down-regulation by mediating Rac3 ubiquitination and degradation

Figure 4

Lysine 166 within Rac3 is an ubiquitin acceptor site for FBXL19 - A. MLE12 cells were co-transfected with FBXL19-V5 wild type and Rac3 lysine mutant plasmids as indicated. Cell lysates were analyzed by immunoblotting with antibodies to V5 tag and β-actin. Rac3-V5 levels were quantified by Image J software. B. MLE12 cells were transfected with Wt Rac3-V5 or Rac3K166R-V5 plasmid. Cell lysates were subjected to immunoprecipitation with an ubiquitin antibody, followed by V5 tag immunoblotting. Input lysates were analyzed by immunoblotting with V5 tag and β-actin antibodies. All the blots are representative of three independent experiments.

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Molecular Cancer

ISSN: 1476-4598