Figure 1

Biopanning enrichment of immunogenic tumor-associated proteins. To confirm the enrichment of the biopanning, the outputs of biopans 1–4 (BP1-BP4) were plated onto LB-Agar plates in limiting dilution and plaque lifts were performed. (A). the output of BP3 revealed an increasing number of immunoreactive phage clones than (B). BP4, and illustrates the ability of the sequential biopans to enrich the concentration of tumor-associated proteins recognized by antibodies in patient serum. To confirm the specificity of enriched phage proteins, two nitrocellulose members were lifted twice on the same LB-Agar plate of BP4. One membrane was probed with pooled AAH/SCD patient sera (D) while the other was probed with pooled normal sera (C). Numerous immunoreactive clones show on the membrane incubated with patient sera (D) that are not seen in the identical membrane incubated with normal serum (C).