Figure 4

Transactivation of VEGF promoter induced by RBP2. (A) Schematic structure of pGL3-VEGF promoter and pGL3-VEGF promoter mutant. (B) After transfection of RBP2 expression vector for 48 h, luciferase activity of VEGF promoter reporters as compared with the control vector and RBP2 siRNA cotransfection for 72 h as compared with control siRNA. *P < 0.05 vs control vector. #P < 0.05 vs control siRNA. (C) The luciferase activity of VEGF promoter mutant reporters with RBP2 expression vector and RBP2 siRNA cotransfection. (D) RBP2 occupancy and trimethylation status of H3K4 at the promoter of the VEGF gene in BGC-823 cells. C and R: Control siRNA and RBP2 siRNA, respectively. Data are mean ± SEM of 3 independent experiments.