Figure 4

E2F1 knockdown enhances cell adhesion through ICAM-1. A. DU145/sh-Con and DU145/sh-E2F1 cells were pre-incubated with anti-ICAM-1 antibody or transient transfected with duplex siRNA of ICAM-1. PBMC labeled with Calcein-AM were added to the indicated cells. After washing three times, attached PBMC were captured using a fluorescence microscope at nine randomly selected fields of each well. B. The number of attached PBMC in the (A) was presented by the bar graph (white bar, DU145/sh-Con; gray bar, DU145/sh-E2F1). The data are shown as the mean value ± SD of triplicate measurement. The stars indicate the significant differences (P < 0.05). C. RT-PCR was employed to validate the efficiency of the siRNA-ICAM-1 in DU145/sh-Con and DU145/sh-E2F1 cells that were used in (A).