Figure 2

ABT-263 upregulates Mcl-1 at both mRNA and protein levels. (A) PLC and Huh7 cells were treated with various doses of ABT-263 for 24 h or with 5 μM ABT-263 for indicated times. Then RT-PCR was performed to detect the level of Mcl-1 mRNA, taking β-actin as a control. (B) HCC cells were treated with 5 μM ABT-263 or vehicle DMSO for 24 h, then qPCR was performed to quantitate Mcl-1 mRNA, taking β-actin as a control. Data were represented as mean ± SD from three independent experiments. (*: P < 0.05). (C) HCC cells were treated as described in (A), then the level of Mcl-1 was analyzed by Western blot, taking α-tubulin as a loading control.