Figure 3
From: Melanoma cells influence the differentiation pattern of human epidermal keratinocytes
Keratinocytes co-cultured with BLM cells exhibit accumulation of keratin 14-positive cells (A, K14, red signal) on the periphery of large colonies exhibiting keratins (green signal). This observation contrasts with the finding in co-cultures of keratinocytes with HPM cells (B). The measurements of the gradients of expression of K14 in both experimental situations (A1, B1) correlate with this observation. In case of keratinocytes co-cultured with BLM cells, this observation is further supported by the measurements of fluorescence intensity index (A2).