Figure 5
From: Melanoma cells influence the differentiation pattern of human epidermal keratinocytes
Detection of K14 (red signal) in colonies of normal human keratinocytes (Control, A), and after the application of FGF-2 (B), VEGF-A (C), IL-8 and CXCl-1 (D), and simultaneous application of all tested substances (E). Green signal represents keratin, bar denotes 50 μm. Panel (F) shows the difference of the thickness of K14-positive layer cells surrounding the keratinocyte colonies. All stimulated cell colonies have significantly different margin extent when compared to control colonies. The cells stimulated simultaneously by all factors have significantly wider margin than the cells treated by individual factors (p < 0.01). The peripheral cells positive for K14 are presented in higher magnification.