Skip to main content
Figure 2 | Molecular Cancer

Figure 2

From: Interfering with TGFβ-induced Smad3 nuclear accumulation differentially affects TGFβ-dependent gene expression

Figure 2

SB inhibitors deregulate TGFβ induced Smad3 nuclear accumulation. A, Western Blotting analysis of whole cell and nuclear extracts of cells treated with 5 ng/ml TGFβ for 30 min after 45 min preincubation with DMSO or SB-202190 (10 μM) and SB-203580 (20 μM). Smad3 protein was detected using a mouse monoclonal Smad3 antibody from Santa Cruz. B, Western Blotting analysis as in A using nuclear extracts of cells stimulated with TGFβ for the indicated times after 45 min preincubation with DMSO or SB-203580 (20 μM). C, same as in A with inhibitors SB-203580 (20 μM), PD98059 (40 μM), Y-27632 (20 μM) or PMA (25 nM), respectively. Coomassie staining and reprobing of blots with a control antibody (PKCα) ensured equal protein loading for all lanes (not shown).

Back to article page

Molecular Cancer

ISSN: 1476-4598