Figure 4
![Figure 4](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2F1476-4598-2-24/MediaObjects/12943_2003_Article_33_Fig4_HTML.jpg)
Effect of demethylation and acetylation on gene expression. Note the lack of reactivation of gene expression in C-4I in RARB gene. Lane 1, untreated; Lane 2, butyrate-treated; Lane 3, 5-Aza-2' Deoxycytidine + butyrate treated, and Lane 4, 5-Aza-2' Deoxycytidine-treated. Genes are shown on left; cell lines are indicated below; Empty arrows indicate the PCR product of actin gene used as control and filled arrowheads represent the indicated gene.