Figure 2
Phosphorylation and immobilization of proteins associated with focal adhesions. Cytoskeletally anchored αVβ6 was immunoprecipitated after TGFβ1 stimulation (10 nM for 10 minutes) followed by Western analysis with antibodies against tyrosine-phosphorylated proteins or αV integrin. In part the cells were preincubated with αV- and β8-, β6-, β5-, β3-, and β1-antibodies (1:100 each for 30 min, all from Santa Cruz) or with a TGFβ1 antibody (15 μg/ml for 30 min, from Santa Cruz) [10].