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Figure 10 | Molecular Cancer

Figure 10

From: p8 inhibits the growth of human pancreatic cancer cells and its expression is induced through pathways involved in growth inhibition and repressed by factors promoting cell growth

Figure 10

p8 transcription is induced by the p38 pathway. Panc-1 and BxPc-3 were cultivated in 30 mm diameter culture dishes for 24 hours, then transiently transfected with 0.5 μg of p8-CAT and 0.5 μg of pCMV/βgal plasmids. The expression plasmid pCEFL HA p38 encoding the wild-type p38 (0.5 μg) was co-transfected with p8-CAT and pCMV/βgal plasmids as indicated. Cell extracts were prepared 24 hours after transfection and CAT and β-galactosidase activities were measured. CAT activity was normalized to β-galactosidase activity. Experiments were carried out in triplicate and repeated two times.

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Molecular Cancer

ISSN: 1476-4598