p8 transcription is induced by the p38 pathway. Panc-1 and BxPc-3 were cultivated in 30 mm diameter culture dishes for 24 hours, then transiently transfected with 0.5 μg of p8-CAT and 0.5 μg of pCMV/βgal plasmids. The expression plasmid pCEFL HA p38 encoding the wild-type p38 (0.5 μg) was co-transfected with p8-CAT and pCMV/βgal plasmids as indicated. Cell extracts were prepared 24 hours after transfection and CAT and β-galactosidase activities were measured. CAT activity was normalized to β-galactosidase activity. Experiments were carried out in triplicate and repeated two times.