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Table 2 Oligonucleotide primers used for MSP

From: CHK2 kinase expression is down-regulated due to promoter methylation in non-small cell lung cancer

Wild Type forward (-615) CCTCC C GGGCTCAAG C GATTCTCCT GCCT (-587)
Methylated forward (-615) TTTTT C GGGTTTAAG C GATTTTTTT GTTT (-587)
Unmethylated forward: (-615) TTTTT T GGGTTTAAG T GATTTTTTT GTTT (-587)
Wild type reverse: (-411) GCG CG GGGGT TCACTCCTGT AGTCCCG CA (-439)
Methylated reverse: (-411) ACG CG AAAAT TCACTCCTAT AATCCCG CA (-439)
Unmethylated reverse: (-411) ACA CA AAAAT TCACTCCTAT AATCCCA CA (-439)