Figure 1
From: PTTG/securin activates expression of p53 and modulates its function
Expression of PTTG and p53 proteins in MCF7, HEK293 and PC3 cells transfected with either pcDNA 3.1 (Lane 1) or pcDNA3.1-PTTG cDNA (lane 2). A: Expression of PTTG and p53 proteins was analyzed by western blot analysis using a PTTG- or p53-specific antibody as described in Methods. A β-Actin antibody was used as a control to determine the variation in protein concentration and loading. B: Double immunocytochemical analysis of HEK293 cells for the co-expression of PTTG and p53 proteins. HEK293 cells were transiently transfected with PTTG cDNA. After 48 hours of transfection, the cells were subjected to immunofluorescence analysis using PTTG-specific antiserum and a p53 monoclonal antibody. Panel A: Cells transfected with pcDNA3.1 vector. Panel B: Cells transfected with pcDNA3.1-PTTG cDNA. 1, pre-immune serum; 2, PTTG antiserum; 3, p53-specific monoclonal antibody, and 4, overlay of 2 and 3 showing co-expression of PTTG and p53 proteins.