Figure 3
From: PTTG/securin activates expression of p53 and modulates its function
Mapping of the PTTG interacting region in the p53 promoter sequence. HEK293 cells (open bars) and PC3 cells (solid bars) were co-transfected with various 5' and 3' deleted constructs of p53 promoter and pcDNA3.1-PTTG. The sequence of the p53 promoter construct (p53/-172/-89) containing the c-myc/max binding sequence is shown. The '*' indicates the position of c-myc/max binding sequence. Transfections were performed in duplicate and the results are expressed as mean ± S.E.M of four independent experiments (*, p < 0.05 by Student's t-test). Results are represented as the fold increase compared to control (pcDNA3.1)-transfected cells.