Figure 6
From: PTTG/securin activates expression of p53 and modulates its function
c-myc is essential for activation of p53 expression by PTTG. A: Co-transfection of HEK293 cells with the c-myc dominant-negative (Myc DN) construct, p53 gene promoter and PTTG cDNA. Transfections were performed in duplicate and results are expressed as mean of two independent experiments. B: Western blot analysis of HEK293 cells transfected with either pcDNA 3.1, the c-myc dominant-negative (Myc DN) and/or PTTG cDNA. Expression was detected using specific antibodies. β-Actin antibody was used as a control to determine the variation in protein concentration and loading.