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Table 1 Sequence of different primers used in PCR amplification Construct

From: PTTG/securin activates expression of p53 and modulates its function

Construct

Primer Sequence

p53/-233/-3

Sense 5'-ATGAGCTC GGATTACTTGCCCTTACT-3'

Antisense 5'-TACTCGAG AATCCAGGGAAGCGTGTC-3'

p53/-172/-3

Sense 5'-ATGAGCTC TTGATGGGATTGGGG TTTTC-3'

Antisense 5'-TACTCGAG AATCCAGGGAAGCGTGTC-3'

p53/-108/-3

Sense 5'-ATGAGCTC CAAAAGTCTAGAGCCACCGT-3'

Antisense 5'-TACTCGAG AATCCAGGGAAGCGTGTC-3'

p53/-172/-89

Sense 5'-ATGAGCTC TTGATGGGATTGGGGTT TTC-3'

Antisense 5'-TACTCGAG ACGGTGGCTCTAGACTTT-3'

p53 cDNA

Sense 5'-TAGGATCCGACACGCTTCCCTGGATT-3

Antisense 5'-TAGAATTCACACCTATTGCAAGCAAG-3'

  1. Restriction enzymes SacI and XhoI sequences are underlined.
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Molecular Cancer

ISSN: 1476-4598