Figure 2
From: A CpG island hypermethylation profile of primary colorectal carcinomas and colon cancer cell lines
Promoter hypermethylation in colon cancer cell lines and colorectal primary tumors. Methylation was evaluated by methylation-specific PCR (MSP). A visible PCR product in Lanes U indicates the presence of unmethylated alleles whereas a PCR product in Lanes M indicates the presence of methylated alleles. The upper panel (a) illustrates the methylation status of all the loci analyzed in a MSI cell line (RKO) and a MSS cell line (HT29). The lower panel (b) shows the methylation status of representative primary colorectal tumors. Abbreviations: NB, normal blood (positive control for unmethylated samples); MP, methylated placenta (positive control for methylated samples); neg, negative control (containing water as template); U, lane for unmethylated MSP product; M, lane for methylated MSP product.