Table 5 Primers and probes used for real-time PCR analysis for 5 target genes
Gene | Forward Primer | Reverse Primer | Probe |
|---|---|---|---|
ACTB | TGAGCGCGGCTACAGCTT | CCTTAATGTCACACACGATT | 6FAM5'-ACCACCACGGCCGAGCGG-3'TAMRA |
PCNA | CTGCAGAGCATGGACTCGTC | GTAGGTGTCGAAGCCCTCAGA | 6FAM5'-ACGTCTCTTTGGTGCAGCTCACCCTG-3'TAMRA |
CDKN2A | CATAGATGCCGCGGAAGGT | CCGGAGGTTTCTCAGAGCCT | 6FAM5'-CCTCAGACATCCCCGATTGAAAGAACC-3'TAMRA |
E2F1 | GAACTGAGGCCTGGGTGATTT | CCACCCATGGCTGTCAGTC | 6FAM5'-TCTGTCTCCCTCCCTCACTTTCCCAATAA-3'TAMRA |
RB1 | TTCCAGAAAATAAATCAGATGGTATGTAA | CAGTGGTTTAGGAGGGTTGCTT | 6FAM5'-CAGCACTTCTTTTGAGCACACGGTCG-3'TAMRA |
Primers used for StaRT PCR analysis for 5 target genes | |||
Gene | Forward primer | Reverse Primer for NT | Reverse Primer for CT |
ACTB | GATTCCTATGTGGGCGACGAG | CCATCTCTTGCTCGAAGTCC | CCATCTCTTGCTCGAAGTCCCCATCTCTTGCTCGAAGTCCGCCAGCCAGGTCCAGACGCA |
CDKN2A | GGAGCCCAACTGCGCCGACC | CCTTCGGTGACTGATGATCTAA | CCTTCGGTGACTGATGATCTAAGGAGCCCAACTGCGCCGACCCCCGTGGACCTGGCTGAG |
E2F1 | TGATACCCCAACTCCCTCTA | AAAGCAGGAGGGAACAGAGC | AAAGCAGGAGGGAACAGAGCAAAGCAGGAGGGAACAGAGCACTGCAGGGACCACAGG |
PCNA | GCTCCAGCGGTGTAAACCTGCA | CGTGCAAATTCACCAGAAGGCA | CGTGCAAATTCACCAGAAGGCACGTGCAAATTCACCAGAAGGCATCAACTTCATTTCATAGTCTGA |
RB1 | TTTCAGAAGGTCTGCCAACACCAA | GTGTCCACCAAGGTCCTGAGATCC | GTGTCCACCAAGGTCCTGAGATCCGTGTCCACCAAGGTCCTGAGATCCCATTTCTGCCAGTTTCTGCTGAAA |