Figure 2

Early apoptosis in tumor cells transfected with survivin and survivin 2α. A: Daoy cells were transiently transfected with control vector, survivin, survivin 2α, or a combination of survivin and survivin 2α. Apoptosis was measured using Annexin V/PI staining 24 hours after transfection, in the absence of a cell death stimulus. The results are shown relative to empty vector control. Error bars represent standard deviations from triplicate experiments. Results were adjusted for transfection efficiency based on parallel transfection with a GFP-expressing plasmid. B: Daoy cells were transiently transfected with control, survivin or survivin 2α. Apoptosis was measured by Caspase-3 activity following treatment with vincristine. C: HeLa cells were transiently transfected with control or GFP-tagged survivin 2α. Electron Microscopy analysis of transfected cells shows a representative cell undergoing apoptosis as induced by survivin 2α. Images were taken 12,000× magnification and scale bars are shown.