Figure 1
From: Inactivation of MAP kinase signalling in Myc Transformed Cells and Rescue by LiCl inhibition of GSK3
The effect of LiCl on the levels of active ERK in Myc transformed and non transformed fibroblasts. (A) A time course for the effect of LiCl on the levels of active ERK2 in v-Myc transformed fibroblasts (panel 1). Total ERK levels were not affected and were used as a loading control (panel 2). Panel 3 is a graphical representation of the ERK2 levels in Li+ and K+ treated v-Myc cells after normalisation to ERK2 levels in non-transformed control CEF (identical to CEF transfected with empty vector) growing under normal conditions. (B) A time course for the effect of LiCl on the levels of active ERK2 in c-Myc transformed fibroblasts (panel 1). Total ERK levels were not affected and were used as a loading control (panel 2). Panel 3 is a graphical representation of the ERK2 levels in Li+ and K+ treated c-Myc cells after normalisation to ERK2 levels in non-transformed control CEF growing under normal conditions. (C) A time course for the effect of LiCl on the levels of active ERK2 in non-transformed control CEF (panel 1). Total ERK levels were not affected and were used as a loading control (panel 2). Panel 3 is a graphical representation of the ERK2 levels in Li+ and K+ treated cells after normalisation to ERK2 levels in non-transformed control CEF growing under normal conditions. The same pattern of expression was seen in several independent experiments for all the panels.