Figure 1

Caveolin-1 expression in human pancreatic adenocarcinoma cell lines. Panel A is a comparison of caveolin-1 protein expression in human ductal pancreatic epithelial (HPDE) and 10 pancreatic cancer cell lines. Aliquots of 50 μg total protein were probed by immunoblotting with a monoclonal antibody specific for caveolin-1. Both the α and β isoforms of caveolin-1 were detected. Immunoblot analysis of β-actin served as a loading control. B. Comparison of BxPC-3 and HPAF-II migration in a colloidal gold random migration assay. PC cells were seeded onto coverslips coated with colloidal gold, stimulated by the addition of 10% FBS and allowed to migrate for 16 h at 37°C. Phagokinetic tracks were photographed and areas measured. Invasion was measured by a Matrigel invasion assay using 10% serum as a chemoattractant. C. HPAF-II cells were transiently transfected with GFP-cav-1 or GFP only and their migratory capabilities measured in a blue-fluorescent bead migration assay in a manner identical to what was described for the colloidal gold assay. A representative Western blot demonstrating that ectopic cav-1 protein levels in the HPAF-II cells were similar to the BxPC-3 cell line. Phagokinetic tracks from GFP-expressing cells were imaged and areas measured. GFP-cav-1 cells were significantly less migratory (*p = 0.0032) and less invasive (*p = 0.002) than the controls.