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Figure 4 | Molecular Cancer

Figure 4

From: Pancreatic Stellate Cells (PSCs) express Cyclooxygenase-2 (COX-2) and pancreatic cancer stimulates COX-2 in PSCs

Figure 4

The expression of COX-2 protein in pancreatic stellate cells (PSCs) was increased by cancer conditioned medium (PANC-1 CM). Stellate cells were isolated and cultured in media containing 10% serum for 12 days. Then, following 18-hour culture in 1% serum medium, cells were treated with PANC-1 CM for the indicated times. Upper panels are representative western blots showing the effect of PANC-1 CM on COX-2 expression over two different time-courses. The lower panels show that densitometric analysis western blots from the separate experiments. PANC-1 CM caused a rapid and sustained up-regulation of COX-2 protein, which was maximally increased after 12 hours and remained elevated.

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Molecular Cancer

ISSN: 1476-4598