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Figure 2 | Molecular Cancer

Figure 2

From: Screening and identification of differentially expressed transcripts in circulating cells of prostate cancer patients using suppression subtractive hybridization

Figure 2

Confirmation of differential gene expression in circulating cells of healthy men and PCa patients using semi-quantitative RT-PCR. RT-PCR was performed on individual samples from 8 healthy controls and 12 PCa patients to confirm the SSH results. After sequencing reaction to reveal the identities of a total of 23 clones present in the subtracted libraries, PCR primers were designed (Table 2). β-actin was also amplified from the same samples using a β-actin primer set (BD Bioscinces Clontech) to serve as an internal control for standardizing the quantity of the RNA applied in each reaction. After PCR amplification, aliquots (10 μl) of these PCR products were electrophoresed into 2% agarose gels followed by ethidium bromide staining.

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Molecular Cancer

ISSN: 1476-4598