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Table 2 PCR primers and conditions for detecting levels of mRNA expression in circulating cells of healthy men and patients with PCa

From: Screening and identification of differentially expressed transcripts in circulating cells of prostate cancer patients using suppression subtractive hybridization

Clone I.D.

GenBank Accession No.

PCR primers

cycles

PCa-001

AC132068

5'-AGG AAT AAG TCA CAC CGT GGA-3' 5'-ACC TGT TGG GAC TAG ACG CAT-3'

20

 

nested

5'-TGG TCT GTA ACC CTT AGG AGA-3' 5'-TCT GCC CTT TGA GTC CAA GT-3'

25

Pca-002

AC019106

5'-AGG TCA GCA GAG ATG TCT GT-3' 5'-TAG TCC CCG AGA AAG AAT TA-3'

32

Nrml-001

AC004690

5'-TGA GCA GTT TCT TCA GCC TCA-3' 5'-TGA TAA GTC CAA CCC AAA GGC T-3'

20

 

nested

5'-TAT CTG GGT GAC ACT GGG AAA-3' 5'-AGA GAC CAG CGT AAT ATC CCT-3'

30

Nrml-002

AK095899

5'-AGG TAA AGG AAA CCC CAA CAT GCA-3' 5'-AAC CAA CGA GGT GGC CGA ATC TT-3'

35

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Molecular Cancer

ISSN: 1476-4598