Gene copy number by Southern blot analysis. Relative copy number of nine of the candidate target genes normalized with APOB in 10 sarcomas. The localisation of BACs RP11-110D4 and RP11-195G14 is based on previous contig maps from the Wellcome Trust Sanger Institute (dotted line), since these are removed from the minimal tiling-path in Ensembl. Gene localisations are based on Ensembl and the UCSC Genome Browser, with the genes listed from the centromeric to the telomeric side. Of the genes located in BACs RP11-297K8 and RP11-122G18, only APOA2 is shown. The densitometrically determined levels of amplification are divided into five categories as indicated.