Skip to main content
Figure 5 | Molecular Cancer

Figure 5

From: Early expression of the Helicase-Like Transcription Factor (HLTF/SMARCA3) in an experimental model of estrogen-induced renal carcinogenesis

Figure 5

Immunofluorescence staining of HLTF in renal tumor-derived HKT-1097 cells. Cultured cells were processed for double immunofluorescence staining as described in Materials and methods. A, E: general cell appearance revealed by vimentin immunostaining (FITC immunolabeling); B, F: virtually all cells in the microscopic fields display HLTF immunoreactivity as shown by application of antisera (Texas Red labeling) directed against either HLTFMet123 (ASE2; B) or HLTFMet1 (ART2; F). C, G: cell nuclei immunolabeled with anti-lamin B serum (Texas Red labeling); D, H: use of ASE2 (D) or ART2 (H) (FITC labeling) reveals the nuclear localization of immunoreactive HLTF variants.

Back to article page

Molecular Cancer

ISSN: 1476-4598