Figure 3

Despite being a stable protein, Cdc14A expression can be significantly increased by incubation with the proteasome inhibitor MG132. A) HCT116 colon cancer cells were incubated for different periods of time in the presence of the protein synthesis inhibitor cycloheximide (10 μg/ml) followed by Western blot with anti-hCdc14A (Ab-1) and anti-actin antibodies. B) Human diploid fibroblasts were incubated for different periods of time with the proteasome-inhibitor MG132 (10 μM) followed by western blot with anti-hCdc14A (Ab-1) or anti-p53 antibodies. C) Cells were mock treated or incubated for 16 hours in the presence of MG132 (10 μM) followed by Western blot using anti-hCdc14A (Ab-1) antibodies.