Smyd2 suppresses NIH3T3 cell proliferation. Exponentially grown NIH3T3 cells were transfected with plasmids encoding myc-tagged Smyd2 or myc-tagged Smyd3. Cells, transfected with the empty expression construct (Mock), served as control. All cells were monitored by cell counting using trypan blue exclusion. The inserts show the level of expression of Smyd2-myc and Smyd3-myc at 0 and 144 hours post transfection, demonstrating similar levels of ectopically introduced proteins in the NIH3T3 cell lines used. Whereas ectopically introduced Smyd3 enhanced the proliferation, Smyd2 displayed a negative effect on the growth rate of NIH3T3 cells.