Figure 8

a, b: The relative levels of HIF-1α and phosphorylation of s6K1 following treatment with Rapamycin in SCC-4a and SCC9 cells. a)The level of phosphorylation of s6K1 were determined following treatment of SCC-4a and SCC-9 cells with 25 μM Rapamycin for 30 minutes followed by EGF for 1h and then lysed. The cell extracts were subjected to SDS-PAGE and immunoblotting. b) Cells were pretreated with 25 μM of Rapamycin as indicated for 30 min, and then exposed to EGF, after 1 hour the cells were harvested and the cell extracts subjected to SDS-PAGE and immunoblotting. Each bar indicates the mean and SD of three repeat wells. Representative gel bands are depicted above the bar graph. (*, P < 0.05)