Effects of U0126 and TPA on anchorage independent growth of RD cells. A. RD cells left untreated (C) or treated with U0126 or 10-7 M TPA were tested for growth in soft agar. Colonies were photographed after 14 days. B. Growth curves of adherent and non adherent RD cells left untreated (●) or treated with U0126 (▼) or TPA (○). RD cells were counted at indicated times. The data shown are the mean ± s.e.m. of triplicates of a representative experiment. Similar results were obtained in three experiments for A and two for B.