Figure 1
From: Resveratrol interferes with AKT activity and triggers apoptosis in human uterine cancer cells
Effect of resveratrol (0 (control or Ctrl), 10 and 100 μM) on apoptosis induction in HeLa, HEC-1A, KLE, RL95-2, Ishikawa and EN-1078D cell lines, after a period of 48 hours. A) Hoescht nuclear staining was performed, and apoptotic cells were counted under a microscope. Results, presented as a percentage of apoptotic cells to the total cell count, are mean +/- SEM of three independent experiments. B) Cleavage/activation of caspases-3 was monitored by Western blot. GAPDH was used as a loading control; results are mean +/- SEM of three independent experiments. C) The effect of resveratrol of the levels of pAKT was determined by western blot analysis. GAPDH was used as a loading control. Results are mean +/- SEM of at least two independent experiments. *p < 0.05 compared to control cells (Ctrl).