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Figure 4 | Molecular Cancer

Figure 4

From: Rhodamine-RCA in vivo labeling guided laser capture microdissection of cancer functional angiogenic vessels in a murine squamous cell carcinoma mouse model

Figure 4

The purity of endothelial cell RNA pool is measured by RT-PCR amplification of endothelial cell marker CD31, squamous cell carcinoma epithelial specific gene Keratin 19 as well as the Beta-Actin housekeeping gene from LCM samples. Approximately 200 endothelial cells as well as control epithelial cells were microdissected and captured from each sample RNA was extracted and then amplified. In order to verify the quality and purity of the LCM endothelial RNA samples, custom designed primers for house keeping gene Beta-Actin, CD31D, and two pairs of Keratin 19 (KER19A, KER19 B) were applied to the pool by RT-PCR. The results indicated that the endothelial pool does not contain Keratin 19 mRNA, which is expressed in the epithelial pool.

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