Figure 2

Hypoxia and VHL knockdown enhance CXCR4 expression. (A). SN12C-VHL-KD cells were serum-starved and exposed to either normoxia or hypoxia (94% N₂, 5% CO₂ and 1% O₂) for the times indicated. Changes in gene expression were then determined by real-time quantitative PCR. Fold induction represents increases in CXCR4 expression in SN12C-VHL-KD cells compared to SN12C cells transfected with vector control (SN12C-VC). (B). SN12C-P, SN12C-VC and SN12C-VHL-KD cells were treated with either normoxia or hypoxia as described in (A) for the times indicated and then subjected to Western analysis to examine changes in CXCR4 protein levels.