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Figure 3 | Molecular Cancer

Figure 3

From: MUC1 gene overexpressed in breast cancer: structure and transcriptional activity of the MUC1 promoter and role of estrogen receptor alpha (ERα) in regulation of the MUC1 gene expression

Figure 3

Effect of estrogen and 4-hydroxytamoxifen (4-OHT) on expression of MUC1 isoform specific mRNA in T47D and MCF7 cells analyzed by RT-PCR. Human T47D (clone 10) and MCF7 cells were cultured in DMEM medium with or without estrogen and 4-OHT supplements as described in "Materials and Methods". Total RNA was extracted and MUC1 isoform specific RT-PCR was performed. Lane M-DNA marker; lane 1 – cells grown in the medium without estrogen; lanes 2, 3 and 4 – cells grown with 0.1 nM, 1 nM and 10 nM estrogen, respectively; lanes 5, 6 and 7 – cells grown with 10 nM estrogen and with 10 nM, 100 nM and 1 μM 4-OHT supplements, respectively.

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