Drug sensitivity of CD133 positive cancer stem cells derived from No. 1049. Both CD133+ and CD133- tumor cells were collected from No. 1049 by FACS sorting. 1 × 104 cells/well were plated in 96-well plate and treated with various concentrations of temozolomide, carboplatin, Taxol and VP16 for 48 hours in 10% FBS/DMEM/F-12 medium. * indicates p < 0.05 compared to autologous CD133- cells. Data are representative of two independent experiments.