Skip to main content
Figure 11 | Molecular Cancer

Figure 11

From: Tumor cell invasion of collagen matrices requires coordinate lipid agonist-induced G-protein and membrane-type matrix metalloproteinase-1-dependent signaling

Figure 11

siRNAs targeting MT1-MMP reduce protein levels and MMP-2 activity on gelatin zymography. (A) Invading HT1080 cells lysates were prepared for Western blot analysis and probed for MT1-MMP and Lamin A/C to demonstrate siRNA efficiency and specificity. Actin was used as a loading control. (B) Conditioned media from siRNA-treated HT1080 cells were collected after 48 hours of invasion and gelatin zymography was performed. Arrows denote pro-MMP-9, and pro- and active forms of MMP-2.

Back to article page

Molecular Cancer

ISSN: 1476-4598