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Figure 6 | Molecular Cancer

Figure 6

From: Tumor cell invasion of collagen matrices requires coordinate lipid agonist-induced G-protein and membrane-type matrix metalloproteinase-1-dependent signaling

Figure 6

HT1080 cell motility using modified Boyden chambers does not require MMPs. HT1080 cells were seeded onto porous (8 μm) polycarbonate membranes coated with type I collagen (1 mg/ml) and allowed to migrate for four hours in the presence of 1 μM LPA or S1P in the presence or absence of synthetic MMP inhibitors (5 μM GM6001, 5 μM TAPI-0, or 5 μM TAPI-1). Membranes were removed, stained, and migrating cells were quantitated using Scion® software and Microsoft Excel®. Data are expressed as mean number of migrating cells × 103 (± S.D.) and represent the results of quadruplicate experiments.

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Molecular Cancer

ISSN: 1476-4598