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Figure 4 | Molecular Cancer

Figure 4

From: Valproic acid and butyrate induce apoptosis in human cancer cells through inhibition of gene expression of Akt/protein kinase B

Figure 4

Constitutively active Akt counteracts butyrate-induced apoptosis. (A) Ovarian carcinoma A2780S cell lines stably integrated with an expression plasmid for constitutively active Akt (A-caAkt) or an empty vector (A-vector) were used for flow cytometry analysis of cells stained with propidium iodide after 16 or 24 hours exposure to sodium butyrate (NaB, 5 mM). The percentage of cells in the subG1 population is indicated in the corresponding graphs. Untreated cells were used as controls. (B) Equal amounts of whole cell extracts (50 μg) were used for Western blot analysis of Akt, phospho-Akt and casepase-3 levels in the A-caAkt and A-vector cells following butyrate treatments. The blot was then stripped and reprobed for loading control (Ctl) with a SRC antibody.

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Molecular Cancer

ISSN: 1476-4598