Figure 5

SiHa cell survival is not affected by valproic acid and butyrate. (A) Flow cytometry analysis of propidium iodide uptake of the SiHa cells following exposure to valproic acid (VPA, 2 mM) or sodium butyrate (NaB, 5 mM). Untreated SiHa cells were used as control and the time of treatments was for 16 or 24 hours. (B) Equal amounts of whole cell extracts (50 μg) were used for Western blot analysis of endogenous Akt and phospho-Akt of the SiHa cells following treatment with valproic acid or butyrate for 16 or 24 hours. The blot was then stripped and reprobed for loading control (Ctl) with a SRC antibody. (C) and (D) Following 16 hours of valproic acid or butyrate treatment, the mRNA levels of Akt1, Akt2 and Akt3 in the SiHa cells were examined by quantitative real-time RT-PCR analysis. 18S was used as an internal control. Results show fold variations of treated cells compared to untreated controls. Error bars represent standard deviations of three independent experiments.