Figure 1
From: Rb induces a proliferative arrest and curtails Brn-2 expression in retinoblastoma cells
Effect of retroviral Rb transduction on Y79 proliferation. A. Structure of the MSCV-GFP retroviral vector and Rb derivatives. Rb is expressed from the MSCV LTR, and GFP from a PGK promoter. B. Western analysis of Rb expression 3 days after infection. C, D. Prevalence of GFP+ cells after infection with the indicated vectors, relative to that at 44 h after infection, displayed as a time course (C) or at day 12 for a separate experiment (D). E. Effect of Rb on cell cycle distribution 84 h after infection. F. Effect of Rb on cell size. Cells infected with MSCV-GFP or MSCV-GFP-Rb were identified by GFP fluorescence and stained for Rb (left) or measured for forward scatter (FSC) by flow cytometry (right). For C-E, infections were in triplicate, data points represent averages, and error bars indicate standard deviation.