Figure 2
From: Identification of candidate methylation-responsive genes in ovarian cancer
Methylation analysis of genes upregulated after 5-aza-dC treatment of the OVCAR-3 cell line. Summary of sodium bisulfite sequencing analysis of CGIs associated with the EMP3, CST6, CAV1, MAGEA3 and CXCL6 genes in 5-aza-dC treated and untreated (control) OVCAR-3 cells. Five clones were sequenced for each sample, and each circle displays the percent methylation of all clones for a single CpG dinucleotide (open circle, 100% unmethylated; filled circle, 100% methylated). Average percent methylation across all CpG sites for untreated (control) and 5-aza-dC treated cells is shown next to each row of circles. (Vertical bars, CpG dinucleotides; arrow, position of the transcription start site; horizontal black rectangle, region amplified and sequenced after sodium bisulfite).