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Figure 10 | Molecular Cancer

Figure 10

From: Attenuation of PTEN increases p21 stability and cytosolic localization in kidney cancer cells: a potential mechanism of apoptosis resistance

Figure 10

Stabilization of p21 in PTEN-knockdown cells iseabrogated by PI3K inhibition with LY-294,002. a. Wild type ACHN cells as well as those stably transfected with PTEN-shRNA or empty vector were incubated with LY-294,002 (10 μM) for the times indicated in the presence of cycloheximide (CHX; 20 μg/ml) for the times indicated, and cell lysate was immunoblotted with antibodies against p21 or β-actin. b. A graphical representation of the data using densitometry of p21 and actin bands is reported as ratio of p21/β-actin and is normalized to the "0 CHX control" for each lane. A representative experiment is shown of three done.

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Molecular Cancer

ISSN: 1476-4598