Nidogen 1 and 2 gene promoters are aberrantly methylated in human gastrointestinal cancer

Table 1 NID1 and NID2 oligonucleotides for MSP and RT-PCR analyses

Gene	Method	Primer 1	Oligonucleotide sequence^a	Primer 2	Oligonucleotide sequence	Product size	T (°C) annealing
NID-1	MSP methyl	NID1_MF	GAGGGTTTCGTTTCGTTTAGC	NID1_MR	AACGCCGTTCGCTAAAAATCG	144	58°C
NID-2	MSP methyl	NID2_MF	TAGTATTGGTAACGACGATAGTATC	NID2_MR	AAATTCGAAACTAACGCGACACG	141	58°C
NID-1	MSP unmethyl	NID1_UF	GAGGGTTTTGTTTTGTTTAGT	NID1_UR	AAAAACACCATTCACTAAAAATCA	147	51°C
NID-2	MSP unmethyl	NID2_UF	TAGTATTGGTAATGATGATAGTATT	NID2_UR	AAATTCAAAACTAACACAACACA	141	51°C
NID-1	RT-PCR	NID1_181F	AGGAGCTCTTTCCCTTCGGC	NID1_354R	CGGGGGTTCACTCGTAGCAA	174	60°C
NID-2	RT-PCR	NID2_722F	TAGGCGCTTACGAGGAGGTCAA	NID2_813R	TATCAGACCCATCAGATGCCAAAAC	92	63°C

^a All primers for MSP were designed on predicted bisulfite-modified DNA sequences (see Additional files 3 and 4)

ISSN: 1476-4598