Differention associated gene expression changes in LADY tumor development. 4A: Differentiation gene expression analysis in control CD-1 and LADY CG at six6 and 16 weeks of age A: Probasin, B: NPDC-1, C: FoxA1. 4B: Foxa1 staining in Control and LADY CG. Panels A and B: FoxA1 protein is detected in the epithelial cells of six and 16 week CG. Panels C and D: Dysplastic epithelial cells continue to express high levels of Foxa1, asteric denotes areas of normal prostatic tissue.