Figure 4
From: A novel small molecule that selectively inhibits glioblastoma cells expressing EGFRvIII
a) Trypan blue studies of cell viability following treatment with NSC-154829. Wild-type (897) or mutant (898) cells were plated and treated for either 24, 48, or 72 hours with 2 μM of NSC-154829. Cell viabilities remain similar until 72 hours when cell death increases dramatically in the 898 cell line compared to similarly treated 897 cells and DMSO-only treated controls. b) Hoechst studies for apoptosis following treatment with NSC-154829. D54 and D54E cells were treated with 2 μM NSC-154829 and then trypsinized, collected and stained with Hoechst 33258 at days 0, 1, 2 and 3. EGFRvIII+ cells showed a consistent and significant increase in the number of apoptotic nuclei compared to wild type.